ARM9131-21C [Monoclonal Antibody]
AIFM1 Rabbit Monoclonal Antibody (Sepharose Bead Conjugate)
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Species:   Rabbit
Applications:   IP
Immunogen Range:   A synthetic peptide corresponding to redidues surrounding Ala520 of human AIF protein
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   9131
Swiss Prot:   O95831
Synonyms:   AIF, CMTX4, COWCK, PDCD8, COXPD6
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7).
Caculated MW:   67 kDa
Observed MW:   Refer to Figures
Applications:  
Reacitivity:   Human, Monkey, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information