AMM4233-21S [Monoclonal Antibody]
MET Mouse Monoclonal Antibody (Flow Specific)
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Species:   Mouse
Applications:   WB IP
Immunogen Range:   human cancer cell lines
Clonality:   Monoclonal Antibody
Isotype:   IgG1
GENE ID:   4233
Swiss Prot:   P08581
Synonyms:   HGFR, AUTS9, RCCP2, c-Met
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).
Caculated MW:   145 kDa
Observed MW:   Refer to Figures
Applications:   FC 1:50
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information