ARP84126P-21
[Polyclonal Antibody]
Phospho-ATRIP-Ser224 Rabbit Polyclonal Antibody
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Species: |
Rabbit |
Applications: |
WB |
Immunogen Range: |
A synthetic phoshopeptide corresponding to residues surrounding Ser224 of the human ATRIP protein |
Clonality: |
Polyclonal Antibody |
Isotype: |
/ |
GENE ID: |
84126 |
Swiss Prot: |
Q8WXE1 |
Synonyms: |
DKFZp762J2115, FLJ12343, MGC20625, MGC21482, MGC26740 |
Purification: |
Affinity purification |
Storage: |
Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5).Cyclin dependent kinase 2 (CDK2) may participate in the regulation of DNA damage response and cell cycle control through phosphorylation of ATRIP at Ser224 (6). |
Caculated MW: |
86 kDa |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:1000
|
Reacitivity: |
Human |
For research use only. Not intended for diagnostic or therapeutic use!