ARMS002-21SC
[Secondary Antibody]
Anti-rabbit IgG Mouse Monoclonal Antibody (HRP Conjugate) (Conformation Specific)
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DATASHEET
Species: |
Rabbit |
Applications: |
WB |
Immunogen Range: |
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Clonality: |
Secondary Antibody |
Isotype: |
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GENE ID: |
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Swiss Prot: |
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Synonyms: |
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Purification: |
Affinity purification |
Storage: |
Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem. |
Caculated MW: |
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Observed MW: |
Refer to Figures |
Applications: |
WB 1:2000
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Reacitivity: |
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For research use only. Not intended for diagnostic or therapeutic use!