ARP57805P-21 [Polyclonal Antibody]
Phospho-KIAA1967-Thr454 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IP IF
Immunogen Range:   A synthetic phosphopeptide corresponding to Thr454 of the human DBC1 protein
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   57805
Swiss Prot:   Q8N163
Synonyms:   DBC-1, DBC1
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor α (ERα) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERα expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-α (TNF-α)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-α is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Overexpression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-α-mediated apoptosis.The threonine residue at 454 of DBC1 is phosphorylated in an ATM/ATR-dependent manner in response to DNA damage (6,7).
Caculated MW:   130 kDa
Observed MW:   Refer to Figures
Applications:   IF 1:400
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information