ARM4790P-21S [Monoclonal Antibody]
Phospho-NFKB1-Ser933 Rabbit Monoclonal Antibody (IHC Specific)
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Species:   Rabbit
Applications:   IHC
Immunogen Range:   A synthetic phosphopeptide corresponding to amino acids around Ser933 of NF-kappaB p105
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   4790
Swiss Prot:   P19838
Synonyms:   NFKB1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).Following IKK-mediated phosphorylation of p105 NF-kappaB at multiple sites (Ser921, 923, 927 and 933) on its carboxy-terminus, SCFbeta-TrCP mediated processing produces the 50 kDa active form p50 (12,13).
Caculated MW:   105 kDa
Observed MW:   Refer to Figures
Applications:   IHC 1:150
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information