ARMF056-21
[Monoclonal Antibody]
CAMK2 Rabbit Monoclonal Antibody
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DATASHEET
Species: |
Rabbit |
Applications: |
WB |
Immunogen Range: |
A synthetic peptide surrounding Val184 of human CaMKII-alpha |
Clonality: |
Monoclonal Antibody |
Isotype: |
IgG |
GENE ID: |
815/816/817/818 |
Swiss Prot: |
Q9UQM7
Q13554
Q13557
Q13555 |
Synonyms: |
/ |
Purification: |
Affinity purification |
Storage: |
Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4). |
Caculated MW: |
60, 50 kDa |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:1000
|
Reacitivity: |
Human, Mouse, Rat |
For research use only. Not intended for diagnostic or therapeutic use!