ARMF012P-21C [Monoclonal Antibody]
Phospho-MAP2K1/2-Ser217/221 Rabbit Monoclonal Antibody (Biotinylated)
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Species:   Rabbit
Applications:   WB IP
Immunogen Range:   A synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   5604/5605
Swiss Prot:   Q02750/P36507
Synonyms:   CFC3/CFC4, MEK1/MEK2, MKK1/MKK2, MAPKK1/MAPKK2, PRKMK1/PRKMK2
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
Caculated MW:   45 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
Reacitivity:   D. melanogaster, Human, Monkey, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information