ARP5315P-21 [Polyclonal Antibody]
Phospho-PKM2-Tyr105 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB
Immunogen Range:   A synthetic phosphopeptide corresponding to the sequence surrounding Tyr105 of human PKM2 protein
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   5315
Swiss Prot:   P14618
Synonyms:   PKM2, TCB, PK3, PKM2 pyruvate kinase, muscle, CTHBP, PK2
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2) is an alternatively spliced variant of M1 that is expressed during embryonic development (1). Research studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect (1). When cancer cells switch from the M2 isoform to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies showed that PKM2 is not essential for all tumor cells (4). In the tumor model studied, PKM2 was found to be active in the non-proliferative tumor cell population and inactive in the proliferative tumor cell population (4).Additional studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active, tetrameric PKM2 (5). A PKM2 missense mutation found in cancer cells results in the replacement of Tyr105 by phenylalanine and leads to reduced cell proliferation during hypoxia and tumor growth in nude mice xenografts (5). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (5).
Caculated MW:   60 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
Reacitivity:   Human, Monkey, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information