ARPF052-21
[Polyclonal Antibody]
TGFB1/2/3 Rabbit Polyclonal Antibody
www.yhsbio.com
market@yhsbio.com
support@yhsbio.com
+86-21-54651191
Room 703,Building 6,333# Guiping
Rd.,Xuhui District,Shanghai,China
DATASHEET
Species: |
Rabbit |
Applications: |
WB |
Immunogen Range: |
A synthetic peptide corresponding to a region in the carboxy terminus of TGF-beta1 |
Clonality: |
Polyclonal Antibody |
Isotype: |
/ |
GENE ID: |
7040/7042/7043 |
Swiss Prot: |
P01137/P61812/P10600 |
Synonyms: |
/ |
Purification: |
Affinity purification |
Storage: |
Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).There are three isoforms of TGF-beta designated TGF-beta1, TGF-beta2, and TGF-beta3, which are encoded by distinct genes and are expressed in a tissue specific manner (10). Each of the isoforms are synthesized as larger precursor proteins containing a propeptide region which gets cleaved prior to secretion from the cell. Mature TGF-beta contains two polypeptides linked linked by disulfide bonds forming a protein of about 25 kDa. |
Caculated MW: |
12, 25, 45 to 65 kDa |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:1000
|
Reacitivity: |
Human, Mouse, Rat |
For research use only. Not intended for diagnostic or therapeutic use!