ARMF052-21
[Monoclonal Antibody]
TGFB1/2/3 Rabbit Monoclonal Antibody
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DATASHEET
Species: |
Rabbit |
Applications: |
WB |
Immunogen Range: |
A synthetic peptide corresponding to a region in the carboxy terminus of TGF-β1 protein |
Clonality: |
Monoclonal Antibody |
Isotype: |
/ |
GENE ID: |
7040/7042/7043 |
Swiss Prot: |
P01137/P61812/P10600 |
Synonyms: |
/ |
Purification: |
Affinity purification |
Storage: |
Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).Three isoforms of TGF-β, designated TGF-β1, TGF-β2 and TGF-β3, are encoded by distinct genes and are expressed in a tissue specific manner (10). Each isoform is synthesized as a larger precursor protein containing a propeptide region that is removed prior to secretion. Mature TGF-β contains two polypeptides linked by disulfide bonds to form a protein of approximately 25 kDa. |
Caculated MW: |
12, 45-60 kDa |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:1000
|
Reacitivity: |
Human |
For research use only. Not intended for diagnostic or therapeutic use!