ARMM018P-21
[Monoclonal Antibody]
Phospho-Ser/Arg-X-Tyr/Phe-X-pSer Motif Rabbit Monoclonal Antibody
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DATASHEET
Species: |
Rabbit |
Applications: |
WB IP |
Immunogen Range: |
Arg-X-Tyr/Phe-X-pSer-containing peptides |
Clonality: |
Monoclonal Antibody |
Isotype: |
/ |
GENE ID: |
/ |
Swiss Prot: |
/ |
Synonyms: |
/ |
Purification: |
Affinity purification |
Storage: |
Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (6). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (6). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR (6). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. |
Caculated MW: |
/ |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:1000
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Reacitivity: |
Human, Mouse, All |
For research use only. Not intended for diagnostic or therapeutic use!