ARPF051P-21 [Polyclonal Antibody]
Phospho-ZAP70-Tyr319/SYK-Tyr352 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IP IF FC
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   6850/7535
Swiss Prot:   P43405/P43403
Synonyms:   /
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9).
Caculated MW:   70 kDa Zap-70, 72 kDa Syk
Observed MW:   Refer to Figures
Applications:   WB 1:1000
IF 1:100
FC 1:50
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information