ARP11200P-22 [Polyclonal Antibody]
Phospho-CHEK2-Ser33/35 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Ser33/35 of human Chk2
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   11200
Swiss Prot:   O96017
Synonyms:   CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).
Caculated MW:   62 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
Reacitivity:   Human, Monkey
For research use only. Not intended for diagnostic or therapeutic use!
Additional information