Species: | Rabbit |
Applications: | WB IP IF FC |
Immunogen Range: | A synthetic peptide corresponding to the carboxy-terminus of human Calcineurin A (alpha isoform) |
Clonality: | Polyclonal Antibody |
Isotype: | / |
GENE ID: | 5530/5532/5533 |
Swiss Prot: | Q08209/P16298/P48454 |
Synonyms: | / |
Purification: | Affinity purification |
Storage: | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs.Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding. |
Caculated MW: | 59 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:1000 IF 1:50 FC 1:25 |
Reacitivity: | Human, Monkey, Mouse, Rat |