ARP1028P-21 [Polyclonal Antibody]
Phospho-CDKN1C-Thr310 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IF
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Thr310 of human p57 Kip2
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   1028
Swiss Prot:   P49918
Synonyms:   BWCR, BWS, KIP2, WBS, p57
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).Levels of p57 Kip2 are controlled by ubiquitination/degradation via the Skp1/Cul1/F-box-type E3 ubiquitin ligase complex SCF-Skp2, and this effect is dependent on Thr310 (5). A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regulate protein stability (6).
Caculated MW:   57 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
IF 1:100
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information