Species: | Mouse |
Applications: | WB IP IF FC |
Immunogen Range: | A recombinant beta-galactosidase protein |
Clonality: | Monoclonal Antibody |
Isotype: | IgG1 |
GENE ID: | 945006(E.coli) |
Swiss Prot: | P00722 |
Synonyms: | / |
Purification: | Affinity purification |
Storage: | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.Eukaryotic genes can be cloned into E. coli beta-galactosidase (lacZ) gene (1-3). This results in the expression of a fusion protein containing the desired protein with a beta-gal tag. An antibody to this tag can then be used to isolate the fusion protein or detect its expression within cells or tissues. |
Caculated MW: | / |
Observed MW: | Refer to Figures |
Applications: |
WB 1:1000 IF 1:3200 FC 1:7000 |
Reacitivity: | All |