ARM1147P-21 [Monoclonal Antibody]
Phospho-CHUK-Ser176/IKBKB-Ser177 Rabbit Monoclonal Antibody
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Species:   Rabbit
Applications:   WB
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Ser177 of IKKβ
Clonality:   Monoclonal Antibody
Isotype:   /
GENE ID:   1147
Swiss Prot:   O15111
Synonyms:   CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
Caculated MW:   85 kDa (IKKa) 87 kDa (IKKb)
Observed MW:   Refer to Figures
Applications:   WB 1:1000
Reacitivity:   Human, Mouse
For research use only. Not intended for diagnostic or therapeutic use!
Additional information