ARMF033P-22C [Monoclonal Antibody]
Phospho-SMAD2-Ser465/467/SMAD3-Ser423/425 Rabbit Monoclonal Antibody (PE Conjugate)
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Species:   Rabbit
Applications:   FC
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human Smad2 protein
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   4087/4088
Swiss Prot:   Q15796/P84022
Synonyms:   /
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
Caculated MW:   52, 60 kDa
Observed MW:   Refer to Figures
Applications:   FC 1:50
Reacitivity:   Human, Monkey, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information