ORM21926-21S [Monoclonal Antibody]
TNF Rabbit Monoclonal Antibody (Rodent Specific)
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Species:   Rabbit
Applications:   WB IP IF FC
Immunogen Range:   A recombinant mouse TNF-α protein
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   21926(Mouse)/24835(Rat)
Swiss Prot:   P06804/P16599
Synonyms:   DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
Caculated MW:   17,25,28 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
IF 1:400
FC 1:100
Reacitivity:   Mouse
For research use only. Not intended for diagnostic or therapeutic use!
Additional information