ARP10735-11 [Polyclonal Antibody]
STAG2 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   IHC
Immunogen Range:   A recombinant protein corresponding to amino acids of human STAG2
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   10735
Swiss Prot:   Q8N3U4
Synonyms:   DKFZp686P168, DKFZp781H1753, FLJ25871, SA-2, SA2, bA517O1.1
Purification:   Antigen affinity purification
Storage:   Store at 4°C. For long term storage store at -20°C in PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).Avoid freeze/thaw cycles.
Background:   The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2).
Caculated MW:   141 kDa
Observed MW:   Refer to Figures
Applications:   IHC 1:200-1:500
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information