ARP8844-11 [Polyclonal Antibody]
KSR1 Rabbit Polyclonal Antibody
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Species:   Goat
Applications:   WB ELISA
Immunogen Range:   A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human KSR1
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   8844
Swiss Prot:   Q8IVT5
Synonyms:   KSR, RSU2
Purification:   Protein G purification
Storage:   Store at 4°C. For long term storage store at -20°C in PBS, pH 7.2 (0.09% sodium azide).Avoid freeze/thaw cycles.
Background:   KSR1 (kinase supressor of Ras) was identified from a genetic screen in Drosophila and C. elegans as a component of the Ras signaling pathway (1). KSR1 has a putative carboxy-terminal kinase domain that lacks a key Lys residue for phospho-group transfer. Although reports indicate that ceramide and EGF activate KSR1 (2,3), other evidence demonstrates that KSR1 regulates Raf in a kinase-independent manner (4,5). It is now widely accepted that KSR1 functions as a scaffold that binds MEK1/2 and 14-3-3 protein constitutively and binds ERK1/2 in a Ras activation-dependent manner (1,5,6). HSP70/HSP90 and p50 Cdc37 associate with the KSR1 complex to ensure its stability (5). Multiple phosphorylation sites have been identified: Ser297 and Ser392 mediate 14-3-3 binding, and putative MAPK phosphorylation sites include Thr260, Thr274 and Ser443 (6). C-TAK1 (Cdc25C-associated kinase 1) binds and phosphorylates KSR1 at Ser392 in quiescent cells (7). In response to stimuli, Ser392 is dephosphorylated by PP2A, which leads to ERK1/2 association and allows the KSR1 complex to translocate from cytosol to membrane, where the MAPK pathway is activated (8). IMP, a Ras-responsive E3 ubiquitin ligase, is also involved in interaction with KSR1 and may regulate its localization and stability (9). Very high expression levels of KSR1 inhibit MAPK signaling, whereas physiological levels promote MAPK signaling, indicating that the scaffold protein can turn signaling "on" or "off" depending on the scaffold concentration (10).
Caculated MW:   100 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:100-1:500
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information