ARP64784-11
[Polyclonal Antibody]
CRTC3 Rabbit Polyclonal Antibody
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Species: |
Rabbit |
Applications: |
IHC IF |
Immunogen Range: |
A recombinant protein corresponding to amino acids of human CRTC3 |
Clonality: |
Polyclonal Antibody |
Isotype: |
IgG |
GENE ID: |
64784 |
Swiss Prot: |
Q6UUV7 |
Synonyms: |
FLJ21868, TORC3 |
Purification: |
Antigen affinity purification |
Storage: |
Store at 4°C. For long term storage store at -20°C in PBS, pH 7.5 (40% glycerol, 0.02% sodium azide).Avoid freeze/thaw cycles. |
Background: |
Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein CRTC2 (CREB-regulated transcription coactivator 2)/TORC2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, CRTC2/TORC2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of CRTC2/TORC2 and its dissociation from 14-3-3 proteins. Dephosphorylated CRTC2/TORC2 enters the nucleus to promote CREB-dependent transcription. CRTC2/TORC2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). |
Caculated MW: |
76-78 kDa |
Observed MW: |
Refer to Figures |
Applications: |
IHC 1:500-1:1000 IF 1-4 ug/mL
|
Reacitivity: |
Human |
For research use only. Not intended for diagnostic or therapeutic use!