AMMC001-11 [Monoclonal Antibody]
CDH Mouse Monoclonal Antibody
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Species:   Mouse
Applications:   WB IHC
Immunogen Range:   A mixture of synthetic peptides (conjugated with KLH) corresponding to C-terminus of chicken N-Cadherin with an extra N-terminus lysine residue (24 amino acids)
Clonality:   Monoclonal Antibody
Isotype:   IgG1
GENE ID:   999/1000/1001/1002
Swiss Prot:   P12830 P19022 P22223 P55283
Synonyms:   /
Purification:   Affinity purification
Storage:   Store at -20°C on dry atmosphere.After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C or lower.Avoid freeze/thaw cycles.
Background:   Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).
Caculated MW:   130-150 kDa
Observed MW:   Refer to Figures
Applications:   WB 1-2 ug/mL
IHC 2-4 ug/mL
Reacitivity:   Chiken, Human, Mouse, Rabbit, Rat, Snake
For research use only. Not intended for diagnostic or therapeutic use!
Additional information