AMM8678-14 [Monoclonal Antibody]
BECN1 Mouse Monoclonal Antibody
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Species:   Mouse
Applications:   WB IHC ELISA FC
Immunogen Range:   A recombinant protein corresponding to human BECN1
Clonality:   Monoclonal Antibody
Isotype:   IgG1
GENE ID:   8678
Swiss Prot:   Q14457
Synonyms:   ATG6, VPS30, beclin1
Purification:   Affinity purification
Storage:   Store at 4°C. For long term storage store at -20°C in ascites (0.04% sodium azide). Avoid freeze/thaw cycles.
Background:   Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).
Caculated MW:   60 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information