ARP1398P-31 [Polyclonal Antibody]
Phospho-CRK-Tyr221 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   ELISA Dot
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Tyr221 of human CrkII
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   1398
Swiss Prot:   P46108
Synonyms:   CRKII
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3).
Caculated MW:   42 kDa
Observed MW:   Refer to Figures
Applications:  
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information