Species: | Rabbit |
Applications: | WB IHC ELISA IF |
Immunogen Range: | A synthetic peptide corresponding to 16 amino acids at C-terminus of human CRTC2 |
Clonality: | Polyclonal Antibody |
Isotype: | / |
GENE ID: | 200186 |
Swiss Prot: | Q53ET0 |
Synonyms: | RP11-422P24.6, TORC2 |
Purification: | Peptide affinity purification |
Storage: | Store at 4°C. For long term storage store at -20°C in PBS (0.02% sodium azide).Avoid freeze/thaw cycles. |
Background: | Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein CRTC2 (CREB-regulated transcription coactivator 2)/TORC2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, CRTC2/TORC2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of CRTC2/TORC2 and its dissociation from 14-3-3 proteins. Dephosphorylated CRTC2/TORC2 enters the nucleus to promote CREB-dependent transcription. CRTC2/TORC2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). |
Caculated MW: | 80 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1-2 ug/mL IHC 2.5 ug/mL IF 20 ug/mL |
Reacitivity: | Human, Mouse, Rat |