ARM200186-11
[Monoclonal Antibody]
CRTC2 Rabbit Monoclonal Antibody
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DATASHEET
Species: |
Rabbit |
Applications: |
WB IHC ELISA IF |
Immunogen Range: |
A synthetic peptide of human CRTC2 is used for rabbit immunization |
Clonality: |
Monoclonal Antibody |
Isotype: |
IgG |
GENE ID: |
200186 |
Swiss Prot: |
Q53ET0 |
Synonyms: |
RP11-422P24.6, TORC2 |
Purification: |
Affinity purification |
Storage: |
Store at -20°C or lower in PBS(pH 7.4). Avoid freeze/thaw cycles. |
Background: |
Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling. The protein CRTC2 (CREB-regulated transcription coactivator 2)/TORC2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways. In quiescent cells, CRTC2/TORC2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of CRTC2/TORC2 and its dissociation from 14-3-3 proteins. Dephosphorylated CRTC2/TORC2 enters the nucleus to promote CREB-dependent transcription. CRTC2/TORC2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting. CRTC2/TORC2-related proteins CRTC1/TORC1 and CRTC3/TORC3 also act as CREB co-activators. CRTC1/TORC1, CRTC2/TORC2 and CRTC3/TORC3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats. CRTC1/TORC1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions. When these cells are exposed to cAMP or a calcium activator, CRTC1/TORC1 is dephosphorylated and translocates into the nucleus. CRTC1/TORC1 is essential for energy balance and fertility. |
Caculated MW: |
80 kDa |
Observed MW: |
Refer to Figures |
Applications: |
|
Reacitivity: |
Human |
For research use only. Not intended for diagnostic or therapeutic use!