AMM27134-11 [Monoclonal Antibody]
TJP3 Mouse Monoclonal Antibody
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Species:   Mouse
Applications:   WB S-ELISA ELISA
Immunogen Range:   TJP3 (NP_055243, 868 aa ~ 952 aa) partial recombinant protein with GST tag
Clonality:   Monoclonal Antibody
Isotype:   IgG2a Kappa
GENE ID:   27134
Swiss Prot:   O95049
Synonyms:   MGC119546, ZO-3
Purification:   Affinity purification
Storage:   Store at -20°C or lower in PBS(pH 7.4). Avoid freeze/thaw cycles.
Background:   Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton. ZO-1 and -2 are required for tight junction formation and function. In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators. The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer. Exogenous expression of the amino terminal portion of ZO-3 exerts a dominant negative effect that interferes with assembly of tight junctions and adherens junctions. However, additional evidence indicates that tight junctions do form in the absence of ZO-3 protein, and that mice lacking ZO-3 appear to develop normally.
Caculated MW:   140 kDa
Observed MW:   Refer to Figures
Applications:  
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information