AMP9093-11
[Polyclonal Antibody]
DNAJA3 Mouse Polyclonal Antibody
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Species: |
Mouse |
Applications: |
WB |
Immunogen Range: |
DNAJA3 (NP_0051382, 1 aa ~ 480 aa) full-length human protein |
Clonality: |
Polyclonal Antibody |
Isotype: |
/ |
GENE ID: |
9093 |
Swiss Prot: |
Q96EY1 |
Synonyms: |
FLJ45758, TID1, hTid-1 |
Purification: |
Affinity purification |
Storage: |
Store at -20°C or lower in PBS(pH 7.4). Avoid freeze/thaw cycles. |
Background: |
Human Tid-1 is a human orthologue of the Drosophila tumor suppressor lethal tumorous imaginal discs, and is a member of the DnaJ family of proteins that serve as co-chaperones to Hsp70 proteins. These proteins are characterized by a J domain, a highly conserved tetrahelical domain that binds to Hsp70 chaperones and activates their ATPase activity. Hsp70 and their associated chaperones mediate a variety of activities including the folding of newly synthesized polypeptides, the translocation of proteins across membranes and assembly of multimeric protein complexes. Two alternatively spliced variants exist for human Tid-1 ,designated hTID-1s and hTID-1L, both which contain the J domain, localize to the mitochondrial matrix, and co-immunoprecipitate with Hsp70. Expression of Tid-1L increases apoptosis induced by the DNA damaging agent mitomycin c (MMC) and by TNF-alpha, and that activity is dependent on its J domain. In contrast, expression of Tid-1S reduces apoptosis by these agents. Tid-1 orthologues are also found in mouse (mTid-1) and rat (rTid-1). The mouse orthologue was originally identified though its interaction with p120 GTPase-activating protein (GAP), raising the possiblity that Tid-1 helps regulates the confirmation, activity, or subcellular localization of GAP. |
Caculated MW: |
52.8 kDa |
Observed MW: |
Refer to Figures |
Applications: |
|
Reacitivity: |
Human |
For research use only. Not intended for diagnostic or therapeutic use!