AMP3064-11 [Polyclonal Antibody]
HTT Mouse Polyclonal Antibody
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Species:   Mouse
Applications:   WB IHC S-ELISA ELISA
Immunogen Range:   HD (NP_002102, 81 aa ~ 190 aa) partial recombinant protein with GST tag
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   3064
Swiss Prot:   P42858
Synonyms:   HD, IT15
Purification:   Affinity purification
Storage:   Store at -20°C or lower in 50% glycerol. Avoid freeze/thaw cycles.
Background:   Rho family GTPases are key regulators of diverse processes such as cytoskeletal organization, cell growth and differentiation, transcriptional regulation, and cell adhesion/motility. The activities of these proteins are controlled primarily through guanine nucleotide exchange factors (GEFs) that facilitate the exchange of GDP for GTP, promoting the active (GTP-bound) state, and GTPase activating proteins (GAPs) that promote GTP hydrolysis and the inactive (GDP-bound) state. The p190 RhoGAP proteins are widely expressed Rho family GAPs. p190-A has been characterized as a tumor suppressor, and research studies have shown that loss or rearrangement of the chromosomal region containing the gene for p190-A is linked to tumor development. p190-A binds the mitogen-inducible transcription factor TFII-I, sequestering it in the cytoplasm and inhibiting its activity. Phosphorylation of p190-A at Tyr308 reduces its affinity for TFII-I, relieving the inhibition. p190-A can also inhibit growth factor-induced gliomas in mice and affect cleavage furrow formation and cytokinesis in cultured cells. Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB. Cells deficient in p190-B display defective adipogenesis. There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain. Levels of tyrosine phosphorylation are enhanced by Src overexpression. IGF-I treatment downregulates Rho through phosphorylation and activation of p190-B RhoGAP, thereby enhancing IGF signaling implicated in adipogenesis.
Caculated MW:   190 kDa
Observed MW:   Refer to Figures
Applications:  
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information