ARP4846-64 [Polyclonal Antibody]
NOS3 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IHC
Immunogen Range:   A synthetic peptideof human NOS3
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   4846
Swiss Prot:   P29474
Synonyms:   eNOS, ECNOS
Purification:   Affinity purification
Storage:   Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). eNOS is activated by VEGF, and this activation is associated with dephsophorylation of eNOS at serine 113. Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).
Caculated MW:   133 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:500-1:2000
IHC 1:50-1:200
Reacitivity:   Human, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information