AMM5897-61
[Monoclonal Antibody]
RAG2 Mouse Monoclonal Antibody
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Species: |
Mouse |
Applications: |
WB ELISA |
Immunogen Range: |
A purified recombinant fragment of human RAG2 expressed in E. Coli |
Clonality: |
Monoclonal Antibody |
Isotype: |
IgG1 |
GENE ID: |
5897 |
Swiss Prot: |
P55895 |
Synonyms: |
RAG-2, RAG2 |
Purification: |
Affinity chromatography |
Storage: |
Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. |
Caculated MW: |
59 kDa |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:500-1:2000
|
Reacitivity: |
Human |
For research use only. Not intended for diagnostic or therapeutic use!