Species: | Mouse |
Applications: | WB IHC ELISA FC |
Immunogen Range: | A purified recombinant fragment of human RAF1 expressed in E. Coli |
Clonality: | Monoclonal Antibody |
Isotype: | IgG1 |
GENE ID: | 5894 |
Swiss Prot: | P04049 |
Synonyms: | C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1 |
Purification: | Affinity chromatography |
Storage: | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. |
Caculated MW: | 72 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:500-1:2000 IHC 1:200-1:1000 FC 1:200-1:400 |
Reacitivity: | Human |