ARP5925P-03 [Polyclonal Antibody]
Phospho-RB-Ser811 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IF
Immunogen Range:   A phospho specific peptide corresponding to residues surrounding S811 of_+E_human RB
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   5925
Swiss Prot:   P06400
Synonyms:   RB1, RB, pRb, OSRC, pp110, p105-Rb
Purification:   Affinity purification
Storage:   Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background:   The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
Caculated MW:   102kDa
Observed MW:   Refer to Figures
Applications:   WB 1:500 - 1:2000
IF 1:50 - 1:200
Reacitivity:   Human, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information