ARPT007-01 [Polyclonal Antibody]
His-Tag Rabbit Polyclonal Antibody
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Species:   Mouse
Applications:   WB WB IP IF
Immunogen Range:   A synthetic peptide of His tag
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   /
Swiss Prot:   /
Synonyms:   /
Purification:   Affinity purification
Storage:   Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni++- chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.
Caculated MW:   /
Observed MW:   Refer to Figures
Applications:   WB 1:2000-1:5000
IF 1:50-1:500
Reacitivity:   /
For research use only. Not intended for diagnostic or therapeutic use!
Additional information