ARP549-01
[Polyclonal Antibody]
AUH Rabbit Polyclonal Antibody
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DATASHEET
Species: |
Rabbit |
Applications: |
WB IHC |
Immunogen Range: |
A recombinant protein of human AUH |
Clonality: |
Polyclonal Antibody |
Isotype: |
IgG |
GENE ID: |
549 |
Swiss Prot: |
Q13825 |
Synonyms: |
|
Purification: |
Affinity purification |
Storage: |
Store at -20oC (regular) or -80oC (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Background: |
The methylglutaconyl-CoA hydratase, mitochondrial protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92% identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40-kDa precursor protein which is subsequently processed to a 32-kDa mature form. |
Caculated MW: |
36kDa |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:500 - 1:2000 IHC 1:50 - 1:200
|
Reacitivity: |
Human, Mouse, Rat |
For research use only. Not intended for diagnostic or therapeutic use!