ARP9470-01 [Polyclonal Antibody]
EIF4E2 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB
Immunogen Range:   A recombinant protein of human EIF4E2
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   9470
Swiss Prot:   O60573
Synonyms:   4EHP, IF4e, 4E-LP, EIF4EL3
Purification:   Affinity purification
Storage:   Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation. eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex. eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo. Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192. Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. A mammalian cap-binding protein related to eIF4E, 4E Homologous Protein (4EHP), was later identified. 4EHP does not interact with eIF4G and therefore cannot activate translation. Instead, it inhibits the translation of a subset of mRNAs.
Caculated MW:   28 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:200-1:2000
Reacitivity:   Human, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information