ARP81631-01 [Polyclonal Antibody]
MAP1LC3B Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IHC IF
Immunogen Range:   A synthetic peptide of human LC3
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   81631
Swiss Prot:   Q9GZQ8
Synonyms:   LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B
Purification:   Affinity purification
Storage:   Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).
Caculated MW:   15 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:500-1:1000
IHC 1:50-1:100
IF 1:50-1:100
Reacitivity:   Human, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information