Species: | Rabbit |
Applications: | WB IHC IF |
Immunogen Range: | A recombinant protein of human PAX3 |
Clonality: | Polyclonal Antibody |
Isotype: | IgG |
GENE ID: | 5077 |
Swiss Prot: | P23760 |
Synonyms: | PAX3, CDHS,HUP2,WS1,WS3,Paired box protein Pax-3,HuP2 |
Purification: | Affinity purification |
Storage: | Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Background: | Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). |
Caculated MW: | 50kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50- 1:200 |
Reacitivity: | Human, Mouse, Rat |