ARP595-01 [Polyclonal Antibody]
CCND1 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IHC
Immunogen Range:   A recombinant protein of human Cyclin D1
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   595
Swiss Prot:   P24385
Synonyms:   BCL1, D11S287E, PRAD1, U21B31
Purification:   Affinity purification
Storage:   Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).Of the three D cyclins, aberrant expression of cyclin D1 has been associated with many forms of cancer including B cell lymphomas and can directly contribute to oncogenesis by various mechanisms including gene translocation or amplification (2). Cyclin D1 also plays a critical role in mammary tissue maturation (5).
Caculated MW:   34 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:500-1:2000
IHC 1:50-1:200
Reacitivity:   Human, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information