Species: | Rabbit |
Applications: | WB IHC IP IF ICC |
Immunogen Range: | A recombinant protein of human NCF2 |
Clonality: | Polyclonal Antibody |
Isotype: | IgG |
GENE ID: | 4688 |
Swiss Prot: | P19878 |
Synonyms: | NCF2, FLJ93058, NCF-2, NOXA2, P67-PHOX, P67PHOX |
Purification: | Affinity purification |
Storage: | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | The phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The NADPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformational change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558 (1). Defects in p47phox, often resulting from recombination between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease (2-4). Elevated oxidative stress due to increased myocardial NADPH oxidase activity may be a contributing factor in heart failure (5,6). p67phox appears to coordinate assembly of NAPDH oxidase as it associates with multiple subunits as well as the α subunit of heterotrimeric G proteins (7). Mutations in the corresponding p67phox gene are also associated with a form of autosomal recessive chronic granulomatous disease (8). |
Caculated MW: | 60 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 ICC 1:20-1:100 |
Reacitivity: | Human, Mouse, Rat |