Species: | Rabbit |
Applications: | WB IHC IF |
Immunogen Range: | A recombinant protein of human EEF1A1 |
Clonality: | Polyclonal Antibody |
Isotype: | IgG |
GENE ID: | 1915 |
Swiss Prot: | P68104 |
Synonyms: | EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1 |
Purification: | Affinity purification |
Storage: | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3). |
Caculated MW: | 50 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:20-1:100 |
Reacitivity: | Human, Mouse, Rat |