Species: | Rabbit |
Applications: | WB IHC IF |
Immunogen Range: | A fusion protein of human VASP |
Clonality: | Polyclonal Antibody |
Isotype: | IgG |
GENE ID: | 7408 |
Swiss Prot: | P50552 |
Synonyms: | Vasodilator-stimulated phosphoprotein, VASP |
Purification: | Affinity purification |
Storage: | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). |
Caculated MW: | 40 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:500-1:1000 IHC 1:50-1:100 IF 1:20-1:50 |
Reacitivity: | Human, Mouse, Rat |